Coding

Part:BBa_K3832010:Design

Designed by: Hongyu Chen   Group: iGEM21_XJTU-China   (2021-10-21)


pykA (pyruvate kinase II)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 953
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 953
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 606
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 953
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 953
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

E.coli DH5a strain

References